Oxidized low-density lipoprotein (OxLDL) is formed by oxidation modification of low-density lipoprotein (LDL) under the action of multiple complex factors in the body. The conversion of LDL to OxLDL is a key physiological link in the occurrence and promotion of atherosclerosis (AS). In the early stage of AS lesions, when the vascular endothelium is damaged, monocytes in the blood can pass through the endothelial gap and transform into macrophages under the intima. After LDL is oxidized and modified into OxLDL, it is mainly phagocytosed by binding to scavenger receptors on the surface of macrophages, resulting in lipid accumulation in macrophages and forming foam cells. As the disease progresses, foam cells accumulate to form lipid streaks and even lipid plaques. When lipid plaques are ruptured, platelets adhere and aggregate to form thrombi, which in turn leads to unstable angina, acute myocardial infarction and sudden death. Studies have found that patients with coronary artery disease and atherosclerosis-related diseases (such as stroke, carotid artery plaque, etc.) have higher OxLDL levels, indicating that OxLDL level detection has positive clinical significance in monitoring the condition of coronary heart disease and atherosclerosis-related diseases. Therefore, this indicator can be used to assess the risk of cardiovascular events. This kit is suitable for in vitro quantitative determination of the content of oxidized low-density lipoprotein in human serum and plasma. Applicable models: Mindray BS240, BS800 fully automatic biochemical analyzer, Hitachi HITACHI 7180, 7170, 7600, 008AS fully automatic biochemical analyzer, Beckman BECKMAN AU5800 fully automatic biochemical analyzer. Detection range: The detection range of this kit is [15.00, 120.00] U/L. Precision: Repeatability ≤10.00%; inter-batch difference ≤15.00%. Analytical sensitivity: When testing 30.00U/L quality control products, the difference in absorbance value should not be less than 0.010. Blank limit: should not be greater than 8.00U/L. Interference test: Endogenous interfering substances in the sample with triglycerides below 10mg/ml, hemoglobin below 10mg/ml and bilirubin below 0.2mg/ml will not affect the test results. Potential cross-reactive substances such as high-density lipoprotein (100mg/dL), low-density lipoprotein (200mg/dL), and very low-density lipoprotein (200mg/dL) will not affect the test results of this reagent.