How to avoid nonspecific adsorption when using magnetic quantitative immunoassay analyzer to detect human body fluid samples?
Publish Time: 2025-04-02
It is crucial to avoid nonspecific adsorption when using magnetic quantitative immunoassay analyzer to detect human body fluid samples. Nonspecific adsorption may lead to false positive results, high background signals and low relative intensity, which may affect the accuracy and sensitivity of the test.
1. Selection and processing of magnetic beads
High-quality magnetic beads: Use high-purity and well-dispersed magnetic beads to reduce nonspecific adsorption caused by the magnetic beads themselves.
Surface modification: Chemical or biological modification of the surface of magnetic beads, introduction of hydrophilic groups or specific ligands to reduce hydrophobic effects and reduce nonspecific adsorption. For example, functional groups such as carboxyl, amino, and hydroxyl groups are introduced to bind to biomolecules through covalent coupling or electrostatic effects to improve the specificity and stability of the binding.
2. Sample processing and dilution
Sample pretreatment: Perform appropriate pretreatment of the sample before testing, such as centrifugation, filtration, etc., to remove impurities and particulate matter in the sample and reduce the possibility of nonspecific adsorption.
Dilute the sample: If the sample concentration is too high, it may lead to increased nonspecific adsorption. Therefore, appropriately diluting the sample can reduce the risk of nonspecific adsorption.
3. Optimization of detection conditions
pH adjustment: Adjust the pH of the sample and buffer to keep it within the range where the biomolecules are stable and nonspecific adsorption is minimized.
Ionic strength control: Control the ionic strength of the reaction system by adding an appropriate amount of salts or other electrolytes to reduce nonspecific adsorption caused by electrostatic effects.
4. Use of blocking agents
Application of blocking agents: Use blocking agents (such as bovine serum albumin, casein, etc.) to block nonspecific sites on the surface of magnetic beads to hinder nonspecific adsorption. The blocking agent can bind to the nonspecific binding sites on the surface of the magnetic beads, thereby reducing the risk of nonspecific adsorption.
5. Experimental design and operating specifications
Experimental design: When designing the experiment, an appropriate control group should be set up to verify the degree and influencing factors of nonspecific adsorption.
Operating specifications: Strictly abide by the experimental operating procedures to avoid contamination and errors during the operation and ensure the accuracy and reliability of the test results.
In summary, by selecting appropriate magnetic beads, optimizing sample processing and dilution, adjusting detection conditions, using blocking agents, and standardizing experimental design and operation, the nonspecific adsorption problem of magnetic quantitative immunoassay analyzer when detecting human body fluid samples can be effectively avoided. These measures help improve the accuracy and sensitivity of the test and provide more reliable data support for clinical diagnosis.
